bs 1448r  (Bioss)

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 interacted with Wnt2 and β-catenin in POF mice. ( A ) qPCR was used to detect Wnt-2 and β-catenin mRNA expressions in the ovarian tissues. ( B ) Immunohistochemistry was applied to measure Notch2 protein expression in the ovarian tissues. Magnification×200, scale bar = 100 μm. ( C ) Western blot analysis was utilized to test Notch2 pathway-related protein expressions (Notch2, Jag1 and Hes2) and Wnt2/β-catenin pathway-related protein expressions (Wnt2, β-catenin, Axin2 and LEF1) in the ovaries. ( D ) Co-immunoprecipitation (Co-IP) was conducted to further verify the protein-protein interactions between Notch2 and Wnt2. P < 0.05 and P < 0.01 vs. Control; # P < 0.05 and ## P < 0.01 vs. oe-NC; @ P < 0.05 and @@ P < 0.01 vs. oe-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Immunohistochemistry, Expressing, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein-Protein interactions, Control

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 acted on the Wnt2/β-catenin pathway in POF cells. ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. P < 0.05 and P < 0.01 vs. Control; * P < 0.05 and ** P < 0.01 vs. Model; # P < 0.05 and ## P < 0.01 vs. sh-NC; @ P < 0.05 and @@ P < 0.01 vs. sh-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Western Blot, Control

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: βcatenin knockdown inhibited Wnt2/β-catenin pathway in POF cells treated with sh-Notch2 and SKL2001 ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. # P < 0.05 and ## P < 0.01 vs. sh-Notch2 + SKL2001 + sh-NC. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Knockdown, Western Blot

Journal: Drug Design, Development and Therapy

Article Title: Combination of Sodium Cantharidinate with Cisplatin Synergistically Hampers Growth of Cervical Cancer

doi: 10.2147/DDDT.S282777

Figure Lengend Snippet: SC inhibits the growth as well as drug resistance of CC cells in vivo. ( A ) process diagram; ( B ) growth curves of tumors; ( C ) weight of tumors; ( D – G ) immunohistochemical staining of the number of positive cells for KI67 ( D ), PTPN1 ( E ), phos-PI3K ( F ), and phos-AKT1 ( G ) in xenograft tumors; ( H ), the proportion of apoptotic cells in tumors determined by TUNEL staining. The experiments were repeated at least three times. N = 6. The data are expressed as the means ± SD of three experiments. Statistical analysis was performed utilizing the one-way (panel C – H ) or two-way ANOVA (panel B ) test combined with Tukey’s test. ** p < 0.01 vs mice injected with Caski-1 + PBS; ## p < 0.01 vs mice injected with Caski-1/R + PBS.

Article Snippet: The membranes were sealed with 5% skim milk for 90 min at ambient temperature, followed by incubation with primary antibodies (1:1000 dilution) to PTPN1 (MABS197, Millipore), phos-PI3K (ab182651, Abcam), and phos-AKT1 (#bs-1448R, Bioss Biotech, Beijing, China) at 4°C overnight and with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:10,000 dilution; Proteintech, Chicago, IL, USA) for 60 min at ambient temperature.

Techniques: In Vivo, Immunohistochemical staining, Staining, TUNEL Assay, Injection